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Among the potential target gene pathways predicted by our bioinformatics analyses (Table 1), we decided to first examine candidate genes involved in neurodevelopment and associated with neurodevelopmental diseases.
In order to examine candidate extract/fractions inhibiting COX-2 in RAW 264.7 cells, ethanol V. heyneana extract (VH) and its fractions (n-hexane-VHH, ethyl acetate-VHE and water-VHW) were tested on COX-2 mRNA expression level by qPCR.
To examine candidate gene expression, 20 primary NPC biopsies and adjacent normal tissue at the resection margins were collected immediately after the surgical resection at Queen Mary Hospital in Hong Kong.
4. To collect and store DNA and examine candidate genes for variation in normal cognitive ageing.
Another approach that has proven successful has been to examine candidate genes based on known genetic phenotypes in other species.
We will check for nonlinear effects of continuous variables, and will examine candidate interactions specified a priori.
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"The focus is to help the American voter to better be able to examine candidates and issues," Mr. Kahle said.
Qualitative methods were informed by a phenomenological research approach to examine candidates' transformative learning experiences in a cultural immersion context.
We chose to examine candidates that had been previously reported to be associated with the developing brain, but not the developing retina, and had commercially available antibodies.
However, we believe that it will be useful to examine candidates' track records of cooperation with residents, relationships with important community members or governors, and recognition of the importance of mutual aid as criteria for selection of VHVs.
Other studies have examined candidate genes, implying that a pathogenetic (and a priori) hypothesis was formulated beforehand.
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