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For histological examinations, sections were stained with haematoxylin and eosin.
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3.4% comprised the "Family Histories" section, while 7.9% comprised the "Physical Examinations" section.
Where consent was given, MRCS results were obtained from the "Assessment and Quality Assurance Examinations" section of the RCSEd.
In addition, 2.8% consisted of the "Laboratory Examinations" section, and less than 1.0% of the annotated sections were the "Radiology Reports".
During post-mortem, examination sections of brain were collected and examined for the WNV presence [ 19].
For tissue morphological examination, sections were stained with hematoxylin-eosin, and 6 separate fields from 4 specimens were analyzed by ImageJ software.
For examination, sections were cut using a freezing microtome.
For gross examination, sections were stained with haematoxylin eosin.
The assessment tool was also revised prior to conducting the endline assessment to address problems with the patient history and physical examination sections identified at baseline.
The importance of brevity and educational value is likely better judged by the family physician whereas history and physical examination sections would be important to both groups.
For collagen IV examination sections were incubated with the primary antibody (goat anti-rat polyclonal antibody, 1 100, 37°C for 60 min; Southern biotech) after blocking with normal rabbit serum (30 min, room temperature).
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