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The grids were allowed to dry for an additional 10 min before examination under the electron microscope.
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Etching with permanganic reagents and examination under the scanning electron microscope revealed that in transverse sections of the unannealed specimens, the legacy of the banded spherulitic morphology is seen at draw ratio 10× but appears to have been overwritten by subsequent developments at 20× and 33×.
Gel specimens were coated with a thin layer of gold using the SC7620 Polaron sputter coater (Quorum Technologies) before examination under the scanning electron microscope (Carl Zeiss EVO LS15).
Ultrathin sections were examined under the electron microscope.
Finally, the grids with sections were washed 6 × 5 min in PBG and were allowed to air dry before examination under the scanning transmission electron microscope.
Examination of the sampled air under the electron scanning microscope highlighted the presence of fragments of glass fibers.
Initial microstructural examination under scanning electron microscopy (SEM) revealed clear differences in pore structure according to the choice of acidic suspension medium.
Although researchers have been studying telomeres for decades, no one had ever isolated them for examination under an electron microscope.
Then, by FIB milling, samples were extracted with an accuracy of localization of 50 nm from specific parts of the microspheres and prepared to a 200 nm uniform thickness film for examination under transmission electron microscopy.
For examination under scanning electron microscope, duodenum sections were fixed using 2.5%% glutaraldehyde in phosphate buffer saline (PBS) (pH 7.4).
Ultra thin sections (60 90 nm) were stained with 2% uranyl acetate and 2% lead citrate prior to examination under a transmission electron microscope.
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