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Because only one tissue sample from surgically resected material was available for examination, the expression of HGF, KGF and their receptors reflects regional disease activity and may be unrepresentative of the entire lung.
Furthermore, on immunohistochemical examination, the expression of fibronectin and integrin α10 in the layered chondrocyte cell sheets verified this adhesiveness and illustrated the specific cartilaginous phenotype of the cell sheets.
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Here, we continue to examine this hypothesis through examination of the expression of the Net receptor frazzled in both Ae. aegypti and An.
Examination of the expression of RFP from the control plasmid, pβactin-RFP, showed that a much greater number of cells were transfected than were labeled by the TRE constructs.
Examination of the expression of imprinted genes have shown that nearly all human ES cell lines possess a substantial degree of stability in terms of genomic imprinting status [30].
For a further examination of the expression of Hb-egf mRNA and its protein in HB-EGF KO and control mice, we performed in situ hybridization analysis and immunohistrochemical analysis respectively (Fig. 1d).
For the examination of the expression of ChR2-Venus in the retina, a few rats of each positive line were perfused with a fixative solution containing 4% paraformaldehyde and 15% saturated picric acid in 0.1 M phosphate buffer (pH 7.2) under deep anesthesia.
We then investigated whether Hh signaling regulates Wnt signaling in swimbladder development by examination of the expression of Wnt target genes, axin1, axin2 and lef1 in two Hh pathway mutants, smob641, in which Hh signaling is completely deprived due to a mutation in the co-receptor gene smoothened [35], and syut4, which is partially deficient in Hh signaling due to shh mutation [36].
Closer examination of the expression of genes from terpenoid metabolism demonstrated a striking biphasic response.
Immunohistochemical examination revealed the expression of u-PA in concordance with CD68-positive cells.
Examination of the expression of 9 selected genes using real-time RT-PCR on unamplified cDNA confirmed differential expression in each case.
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