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Unlike breast tumors, colorectal cancer cells often show an overlapping growth pattern in the examination specimen making FISH analysis less reproducible and harder to interpret.
The variations in the prevalence of infection depend on many factors including age, sexual activity, number of sexual partners, other STDs, phase of menstrual cycle, techniques of examination, specimen collection and laboratory technique.
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For surface examination, specimens were mechanically polished.
In microbiological examination, specimens from all animals, except one, showed positive bacterial growth in catheter and bone samples.
During ultrasonographic examination, specimens were maintained vertically by means of a customized adjustable press simulating the weight bearing position.
Immediately after the radiographic examination, specimens for histological examination were collected by removing each intervertebral disc between the second cervical (C2) and first sacral (S1) vertebra in all of the 20 vertebral columns.
During biomechanical examination, the test specimens were moistened with physiological saline solution every 10 minutes.
For the scanning electron microscope (SEM) examinations, specimens were fixed in 5% glutaraldehyde solution for 24 h and then washed five times in Sodium cacodylate buffer solution (15 min each).
Before the CLSM examinations, specimens were protected from light and incubated at 37°C for 20 min [ 24, 25].
Pathological examination of specimen obtained with EUS-guided fine-needle aspiration biopsy (EUS-FNAB) revealed adenocarcinoma.
Clinical and laboratory assessment includes a pelvic examination with specimen collection.
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