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An evaluation method of AFM tip shape using a probe examination sample and its evaluation criteria are stipulated in the Japanese Industrial Standard (JIS) R 1683: 2007 "Test method for surface roughness of ceramic thin films by atomic force microscopy".
Here, we utilized mouse spinal cord sections as a first examination sample because the distribution pattern of the cholinergic neuron in the spinal cord is simple and well described [ 37].
Prevalence estimates in Tables 1 and 2 were calculated among examined individuals with known diabetes status based on interview and with valid A1C, using examination sample weights; a similar percent of individuals with and without diagnosed diabetes had valid A1C values.
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Prior to examination, samples were platinum sputter-coated.
Prior to examination, samples were sputter-coated with platinum to prevent charging during FESEM imaging.
Prior to examination, samples were gold sputter-coated, to render them electrically conductive.
For histological examination, samples were fixed with 4% paraformaldehyde in a 0.1 M phosphate buffer (pH 7.4) for 3 days.
Before examination, samples were transferred back into LN2.
For SEM examination, samples were sputter coated with gold/palladium and observed with an FEI Quanta 600 (FEI Company, Hillsboro, OR, USA) scanning electron microscope at the Scripps Institution of Oceanography Unified Laboratory Facility.
For the microscopic examination samples of the suspension were directly dripped on the microscope slides.
For histological examination, samples were fixed in 10% (v v−1) formalin and then embedded in paraffin.
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