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At the end of the tested period, all animals were sacrificed and subjected to hematology and clinical chemistry assays, ophthalmic testing (cornea, conjunctiva, iris, pupil, atria, len, fundus, eyelid), necropsy examination, organ weighing, and histopathologic examination.
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Myers et al. (2009) criticized our use of gross examinations, organ wet weights, tissue histopathology, and systemic and reproductive/developmental landmarks because they "were established procedures by the 1950s".
Microscopic examination of organ sections allowed visual identification of HcRed-expressing cells.
Gross examination of organ weight and morphology on all mice necropsied at 2 weeks to 2 years of age.
For virologic examination, homogenized organ tissue was inoculated onto VeroE6/7 cells and monitored daily for cytopathic effects.
Organs from these un-inoculated animals were also used for macroscopic and microscopic comparison in the pathological examination of organs from the experimentally infected animals.
We did not perform post-mortem examinations for organ or vessel damage.
All patients underwent a detailed history with site-specific examinations; pelvic organ prolapse was quantitatively assessed according to the POPQ (pelvic organ prolapse quantitation) staging system.
On completion of the treatment, the animals were bled via the retro orbital plexus and sacrificed by cervical dislocation and necropsied to facilitate gross pathological examination of organs, viz.
Histological examination of organs from knockout mice (Figure 2B) revealed that skin has reduced subcutaneous fat cells in addition to a thickened epidermis and aberrant hair follicles.
Histological examination of organs from MacBlue/UAS-Slfn4-V5 mice revealed an increase in neutrophils in the liver (Figure 7A, arrowheads), as well as an increase in neutrophils and megakaryocytes in the spleens (Figure 7B, arrows).
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