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Oosumi et al. [ 4] did not report the occurrence of tetraploidy among their transformants; however, a careful examination of the four transformants that were photographically documented by these authors suggests to us that the transformant depicted in their Figure eight-b [ 4] may be a tetraploid, as suggested by the distinctive leaf morphology that we have shown to be indicative of tetraploidy.
Upon microscopic examination, about 40% of the transformants showed swimming cells.
A. nidulans aspC complemented the temperature sensitivity of cdc3 and cdc12 mutants (Fig. 2), though microscopic examination of transformants showed cells were still abnormally elongated.
Microscopic examination of S. cerevisiae transformants bearing aspC showed many highly elongated, filament-like cells.
A majority of the transformants resistant to the antibiotic G418 were capable of synthesizing and secreting biologically active human interferon.
Next, we analyzed the growth of the transformants in liquid media using a Cell Growth Quantifier (Aquila Biolabs)39.
Although several of the vector-gpt DNAs can replicate in monkey and mouse cells, none of the transformants contain autonomously replicating vector-gpt DNA.
Multi-copy integration transformants had higher lipid contents than mono-copy integration transformants; the lipid content of the transformants was consistent with the copy number of acl gene integrated.
Southern blot analysis suggested that 87.5 % of transformants obtained with this protocol displayed single hybridization bands, indicating a single insert of T-DNA in each of the transformants.
Over 97% of the transformants retained the sulfonylurea resistance phenotype under non-selective conditions.
To test the inhibition of yeast-expressed GLUT5, variable concentrations (1 50 µm) of MSNBA were added to YEPF media and the growth of the transformants was monitored.
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CEO of Professional Science Editing for Scientists @ prosciediting.com