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Zygosity status of each mutation was determined at the Institute by manual examination of the sequencing electropherograms (response to our query, Sanger #80248).
Ambiguous sites were resolved by reamplifying and resequencing the region using different primer pairs and by examination of the sequencing traces.
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Detail examination of the sequences and the subsequent experiments suggest that the sequenced RNAs are likely to be non-random cleavage products of tRNA.
The well-defined flow field permits a more detailed examination of the sequence of morphological changes during phase inversion than was accomplished previously.
Examination of the sequences of FN and SK indicates a region of homology containing the sequence LTSRPA.
The preceding signatures allow a more detailed examination of the sequence of activity, which we will organize through selected magnetograms (see Fig. 9).
However, examination of the sequences containing the RNA endpoint positions revealed that effectively the only highly conserved regulatory element is the rho-independent terminator that generates the common 3' end.
An examination of the sequences of the ionograms shows that highly-fluctuated STIDs appear at WAK and KOK at 06:00 UT, YAM and OKI at 06 30 UT, and CHL at 06:35 UT.
Discrepancies were verified by manual examination of the sequence traces.
Examination of the sequences with these mutations displayed no evidence for sequence artifact or epidemiological clustering.
Examination of the sequences in the C3 region also revealed several changes (Figure 5c).
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