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Microscopic examination of the lines revealed that, as noted previously for the myo-3PROM-driven transcriptional constructs, the mCitrine and mCherry fusion proteins, even when encoded by CDSs with 2 introns, were, as determined by eye, visibly less bright that the respective F-YFP and Mc-mCherry equivalents.
The disease associated SNP on chromosome 5 was in a sucrose synthase gene (GRMZM2G060659) located in an LD block extending approximately 0.2 Mbp on chromosome 5. Examination of the lines carrying the minor allele at this locus revealed no relationship between population structure due to kernel type (namely the sweet corn and popcorn groups) and presence of the minor allele.
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The most frequently used technique for their unambiguous identification is scanning electron microscopic examination of the lining of the ascospores (25 ).
Examination of these lines indicated that the DIR1 promoter region initiated expression of GUS and DIR1 GUS in seedlings, roots and floral tissues and in all living cells including the veins and mesophyll cells of untreated and mock-inoculated leaves.
All examinations of the line were checked by two midwives increasing the confidence in findings.
Microscopic examination of the raised lines in cross-section showed they are a distinct material in texture and particle size as compared to the brick body and the colored glazes.
Close examination of the spectral lines reveals that some display a fine structure splitting.
In both the examination of the cell lines in vitro as well as the tumour specimens, activated AKT was observed in the absence of HER-2/ neu overexpression.
Whatever the cause(s) the low signal levels made discerning clear expression patterns for the two fusion genes, F09E5.15::mCitrine(2I) and EEED8.6::mCherry(2I), difficult and further examination of the corresponding lines was not pursued.
The results from examination of the mutant lines also suggest that interorganellar transfer of fatty acids are involved in mediating temperature-induced membrane alterations, and reveal steps in the fatty acid unsaturation pathway that appear to have key roles in the acclimatization of membranes to high temperature.
Examination of the test line showed that no major power lines crossed the flight path.
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