Detailed examination of the cell cycle progression of single, unbudded G1 cells for six of these mutants clearly demonstrated that all have cell cycle progression defects associated with G1 or G1/S phase transition.
Examination of the cell cycle and DNA processing categories revealed a prevalence of gene up-regulation from 2 to 16 DAP.
To gain deeper insight into the possible transitions that arose during the emergence of metazoan multicellularity, we have performed a detailed examination of the life cycle and associated transcriptomic changes of Capsaspora owczarzaki, one of the closest known unicellular relatives of metazoans.
Examination of the cell cycle changes that were induced after exposure for 72 h to IC90 for each extract was not performed because at this time point and at this extract concentration low numbers of mostly dead or dying cells were present in the sample.
Each of these enrichment consortia represents a robust ecosystem allowing an examination of the global halogen cycle, where dechlorination is maintained by the collective activities of the microbial community.
During treatment, these assessments were performed in the same manner at the start (Day 1) of each cycle, except radiological examination of the tumor which was performed after Cycle 2 and Cycle 6, and every three months thereafter.
EC was detected in heart, liver and spleen of several broilers at every date of examination until the end of the cycle (Table 1) and was also isolated from the spinal column of one animal with vertebral osteomyelitis.
Although further experiments will be required to elucidate the function of Tb11.NT.28, examination of cell cycle progression following RNAi induction revealed a decreased ability of the cells to divide replicated kinetoplasts.
The antitumor ability of the AND gate genetic circuit in vitro was tested through examination of cell cycle, apoptosis and proliferation rates in A549 cells.
Examination of cell cycle by propidium iodide (PI) staining in multiple independent experiments showed that the percentage of cells that had entered the cell cycle was markedly reduced by Bcl-2 even in p27 −/− cells.
This method allows for examination of cell cycle events in cells progressing normally into a new cell cycle.
