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Although this may seem inconsistent, further examination of the alignment of probes to gene revealed that different probesets for Tsn aligned to different populations of alternatively polyadenylated transcripts.
Examination of the alignment found two sub-populations of sequences that did not fit the overall alignment consensus in these regions (Table S2).
Examination of the alignment as a whole revealed that the PRE was well conserved compared to immediately flanking sequences.
However, examination of the alignment at this position revealed the substitution of residues with unrelated biochemical properties at these positions.
Alignments performed using Bowtie 2 resulted in an even greater number of miRNAs with higher counts; examination of the alignment output revealed that many of these were attributed to the allowance of insertions and deletions.
Examination of the alignment of genes B0240.4 and B0240.2 in C. elegans and gene CBG23278 in C. briggsae (which is the predicted ortholog of B0240.2) suggests that the predicted C. briggsae gene is defective.
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Examination of the alignments, Figure 4, shows that there is a partial homology with the mazG gene at the C terminus, with nucleotides showing some degree of conservation to the Group 1 phage.
The remaining proteins were validated or rejected by visual examination of the alignments.
Careful examination of the alignments of the less conserved proteins confirmed that the low levels of conservation observed in these proteins were not alignment artifacts.
An examination of the alignments with various parameters highlighted the increase in quality of alignment in the later versions of PASS2.3 (2008), which will help in linking representative superfamily members to cover rapidly expanding sequence space.
On detailed examination of the alignments, it was deduced that DUF659 is a part of the fully functional RNase H-like family [ 62] and therefore, no functional inferences could be derived.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com