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We thank Purnomo for the examination of stool samples.
Microscopic examination of stool samples was negative in all patients at initial presentation and during follow up.
On microscopic examination of stool samples 541 (25.6%) of all samples analysed were positive for at least one intestinal parasite.
In the Los Angeles County pilot surveillance system, follow-up, which included examination of stool samples from close contacts, was conducted for 72 patients.
As an alternative method that is particularly useful for examination of stool samples, antigen detection assays using EIA or latex agglutination have been developed [ 160, 161].
The clinical diagnosis of fascioliasis depends largely on the microscopic examination of stool samples for ova by finding eggs with an inconspicuous operculum [ 1, 3, 5].
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A probable case-patient was defined as a person who ate raw perch during the wedding and reported a "tagliatelle-like" worm of varying length in stools, without a history of consumption of raw beef, pork, or other raw fish in the previous 5 years and in the absence of laboratory examination of stool sample.
After successful treatment confirmed by re-examination of stool samples for eggs over a 2 week period, 42 of the original 305 patients were re-infected with Fasciola at varying time intervals during the study period (12 months); 29 (69 %) of these 42 patients were children in the high risk age group i.e. below 12 years (data not shown).
Routine examinations of stool samples and treatment could benefit both the HIV infected and uninfected children in outpatient and inpatient settings.
Pretreatment of stool samples was performed as described above.
NOE participated in the parasitological analysis of stool samples.
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