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The DNA structures of mutant viruses were verified by PCR, DNA sequencing and examination of restriction enzyme digestion profiles of Escherichia coli-derived BAC DNA and PCR of reconstituted virus DNA.
That a pure strain had been isolated was backed up by examination of restriction digest and Southern blot hybridisation patterns for DNA derived from multiple cultures of the Tiberius strain (data not shown) no variation was detected which would have indicated two strains rather than one.
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Similarly, examination of other restriction enzymes with diverse RSs, in addition to the ones examined in this study, promises great potential to identify "gold standard" sets of enzymes for groups of taxa, with the goal of obtaining neutral RAD-seq datasets.
Examination of a restriction fragment length polymorphic (RFLP) site within the 3'untranslated region of the cDNA following reverse transcriptase (RT -PCR (29/53 infoRT -PCR cases) showed a strong association between cytoplasmic staining and imbalance in allele-specific message levels.
An examination of the potential restrictions on speech or association that have been imposed by the regulations under challenge, demonstrates that the restrictions imposed are reasonable, and are consistent with the inmates' status as prisoners and with the legitimate operational considerations of the institution.
Blast searches for restriction enzymes and examination of genes surrounding DNA methylases, identified 21 potential restriction enzymes (see Additional file 6), seven of which were found to be co-localized with putative methylases (see Additional file 7) in the Mic-PCC7806 genome.
On examination of the foetus no growth restriction was noted.
Examination of gastric cancers using the restriction landmark genomic scanning technique revealed methylation at a NotI enzyme site in TCF4 intron 8 and further identified CpG dinucleotide hypermethylation in TCF4 exon 1, strongly associated with gene silencing in gastric cancer cell lines.
The International Classification of Functioning (ICF) framework provides a conceptual model to guide patient assessment, which includes examination of impairment, activity limitations and participation restrictions [ 2, 5].
On examination there was restriction of flexion at the wrist (average 29.16°), and rotations (supination 31.66° and pronation 30.83°).
Food restriction schedule If applied, type of restriction schedule.
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CEO of Professional Science Editing for Scientists @ prosciediting.com