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Critical examination of protein bands in BG-grown culture extract, a significant induction of all proteins was observed and was determined by protein estimation too.
One half of the tumor tissues were immediately snap-frozened in liquid nitrogen and stored at −80°C for later examination of protein phosphorylation by SDS-PAGE and Western blot.
Indeed, the presence of the repeated peptide groups and unique radical groups allows the examination of protein molecules at the level of its primary structure as almost periodic formation of a crystalline type.
These include target-independent screening of tumor-derived cell lines (disease-dependent), reductionist approaches to identifying crucial elements of disease-affected pathways, disease-independent screening of homologs of previously drugged targets, disease-dependent 'global' examination of gene transcript levels, and disease-dependent global examination of protein expression levels.
Examination of protein homology revealed that SCD altered amino acids which were highly conserved across species.
Examination of protein binding partners may allow useful therapeutic targets to be identified.
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Fortunately within the last decade, MS-based proteomics has evolved into a high-sensitivity high-throughput approach for quantitative examination of proteins from any biological system on a global scale (Fig. 1).
Examination of proteins modulated by SmpB revealed a number of SmpB regulated cellular processes.
Most of the proteomics approaches for PDAC have focused on assessment of tissue proteome [25], [26], [27] and to some extent examination of proteins secreted in the pancreatic juice [18], [22], [23], [28].
Thereafter, a thorough examination of protein-coding gene content was performed as follows.
This was followed by a more detailed examination of protein-coding genes, rRNA genes, tRNA genes, introns, and additional features such as sequence repeats.
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