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Bacteriological examination of milk samples were performed free of charge for the farmer.
In addition, bacteriological examination of milk samples before treatment of clinical mastitis is not a common practice in Estonia.
Examination of milk and tissues from animals in Egypt for Brucella spp. showed increased prevalence rates of serologically reactive animals.
Bacteriological examination of milk samples taken before the experiment indicated these animals were not infected with any major mastitis-causing pathogen prior to the experiment.
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All bacteriological examinations of milk samples were performed according to the standards of the National Mastitis Council [ 26].
The milk was macroscopically unremarkable at that time hence a bacteriological examination of the milk was not conducted.
Indeed, a bacteriological examination of the milk in another scrapie-affected ewe that is currently being milked and also presented with a SCC of 161,000 cells and 1,630,000 cells per ml on two subsequent weeks did not show any bacterial growth (T Konold, unpublished observation).
The udders of the cows were pathogen-free prior to the experiment as determined by bacteriological examination of quarter milk samples 1 week before the start of experiment.
B-mode and colour Doppler ultrasonographic examination of the milk vein (subcutaneous abdominal vein) and musculophrenic vein were carried out in all cows on the left and right sides.
Finally, bias associated with intermittent shedding and with early infection were limited by i) repeating bacteriological examinations of individual milk samples at regular intervals and ii) not taking into account milk samples performed during the period of 45 days prior to blood sampling.
Bacteriological examinations of quarter milk samples were performed at the Mastitis Laboratory of the National Veterinary Institute, Oslo, Norway.
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