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A novel bacteriological agar, named Furukawa Agar, has been specifically designed to inhibit the growth of filamentous fungi during microbiological examination of bacteria from organic compost, thereby allowing complete analysis of the resulting bacterial organisms, without the overgrowth with filamentous fungi.
Before experiment, fish were randomly sampled for the examination of bacteria and megalocytivirus in blood, liver, kidney, and spleen as reported previously [ 31, 32].
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Examination of lysed bacteria by electron microscopy showed bacteria with blurred edges surrounded by clumps of lysed debris; bacteria from unlysed samples showed distinct edges against a clear background (Figure 1D).
Examination of adherent bacteria and levels of receptor expression by microscopy revealed that bacteria bound to all transfectants other than sham-transfected cells (Fig. 8).
Nonetheless, data from light microscopic examination of immunostained bacteria pre-incubated with Aβ confirm that the peptide binds to the surface of bacterial cells (Figure 2).
Additional analyses of B. garinii strain 50/97, as well as production and examination of transformed bacteria that specifically express a CRASP-3/CRASP-5/OspE plusein plus CRASP-1 or CRASP-2 will help clarify each CRASP alone and in combination.
The first collected aliquot was used for the microscopic and cultural examination of common bacteria and fungi, and of direct acid-fast bacilli smears (Kinyoun method) and cultures for mycobacteria, and for the microscopic examination of Pneumocystis jiroveci (Gomori-Grocott silver stain).
Examination of any bacteria.
Electronic microscopic examination of negatively stained bacteria indicated that the bacteria expressed type 1 pili (data not shown), and similar titres (1/8) of yeast cell aggregation were obtained compared with the wild-type strain LF82 (figure 4C), indicating that the LF82-Δ ompA isogenic mutant synthesised levels of functional type 1 pili similar to the wild-type strain LF82 (figure 4C).
Study inclusion criteria were suspicion of viral meningitis/meningoencephalitis, a CSF cell count <500 cells/mm, and negative results of culture and microscopic examination for bacteria and fungi.
This study and the others discussed (Chapin et al. 2005; Predicala et al. 2002) examined only culturable bacterial organisms; the lack of examination of other nonculturable bacteria is a limitation of the studies.
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