Results of routine microbiologic examinations for bacteria by culture and antigen detection were negative in all cases, as were serologic and genetic tests for hantaviruses, phleboviruses, and Rickettsiales bacteria, performed as described (10 ).
Analyses were adjusted for age at examination and for bacteria and viruses present in the same sample in the respective analyses of independent effects.
Before experiment, fish were randomly sampled for the examination of bacteria and megalocytivirus in blood, liver, kidney, and spleen as reported previously [ 31, 32].
The diagnosis of meningitis was based on clinical symptoms and cerebrospinal fluid examinations and cultures for bacteria as well as polymerase chain reaction (PCR) for viruses.
At the same time, our results highlight the fact that it is necessary to conduct pathological examinations not only for bacteria but also for viruses and M. pneumoniae for etiological diagnosis of CAP patients.
According to our diagnostic strategy for pulmonary infectious disease in cancer patients with diffuse pulmonary infiltrates, non-invasive tests including blood cultures; examination of the sputum for bacteria, mycobacteria, and fungi; urine tests for antigens of Streptococcus pneumoniae and Legionella pneumophila; and serologic tests for Mycoplasma were performed routinely.
Microbiological examination of BAL fluid was negative for bacteria, while the histopathological examination yielded signs of alveolar damage with alveolar hemosiderosis (Golde score 38).
We took environmental specimens, including samples from the antiseptic solution and the salined cotton, for examination, and bacteria classified as E. amnigenus were cultured from the unused packages of salined cotton in the container that had been used in the blood sampling room for wiping the skin with disinfectant prior to venipuncture for blood culture.
First were 32 samples in which no causal agent was found; pathogens were excluded by routine examination with conventional diagnostic techniques for bacteria, parasites, and norovirus at Southern Community Laboratories.
However, the patient in our study had two aneurysms at the right superficial femoral artery; 4) no history and signs of genital ulcer and skin rash ruled out the Behçet's disease [ 13]; 5) afebrile, unremarkable cardiac examination and negative culture results for bacteria and mycetes demonstrated the impossibility of endocarditis [ 15].
