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Exact(31)
Pubertal assessments were made on the basis of the 5 Tanner stages of classification by physical examination, as described elsewhere (11, 13).
Two weeks later, mice were killed by cervical dislocation and spleens were removed for bacteriological examination as described below.
The brains and eyes were removed, postfixed for 1 2 days, and processed for histological examination as described below.
Incubations were 1 hour at 37°C and were not transferred to new wells before fixing, staining, and examination as described below.
The criteria consisted in the evaluation of (i) the absence of vegetative cells (rods) determined by microscopic examination as described, and (ii) the survival of spores in hydrochloric acid (2.5 N).
The AAV2-infected retinas were removed, stored in a fixative containing 4% paraformaldehyde in 0.1 M phosphate buffer (PB) (pH 7.2), and processed for histological examination as described below.
Similar(29)
The SLN examination was performed as described elsewhere [ 6].
For all patients, a full ear, nose, and throat examination was performed, as described in previous reports.
The CMAPs were recorded in tendon-belly technique and examinations were performed as described elsewhere [ 32].
Immunostaining of filter-grown MDCK cells and microscopic examination were done essentially as described [68].
Pathological examination of the recovered carcase (the first case seen) was performed using a standardised protocol comprising systematic external and internal examination of body systems, as described by [ 15].
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