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Microbial examination was performed only on stool samples that were collected from children during routine clinical examination and analyzed.
Limb length discrepancy, re-fractures, misalignment, range of motion and limitations in daily living were documented through clinical examination and analyzed by the modified Harris-Score [ 23].
Urinary arsenic concentrations were measured in a spot sample collected during the physical examination and analyzed within 3 weeks of collection using high-performance liquid chromatography (HPLC) coupled to inductively coupled-plasma dynamic reaction cell mass spectrometry (ICP-DRC-MS).
An electrocardiogram was also recorded during the in-home examination and analyzed at a central reading center to determine the presence of atrial fibrillation, myocardial infarction, and left ventricular hypertrophy (LVH).
DL fluid was also obtained from 56 asymptomatic high-risk women (Gail risk ≥ 1.7, previous contralateral breast cancer, or carrier of a BRCA1 or BRCA2 gene mutation), in whom there were no suspicious findings on mammography or physical examination, and analyzed for methylated markers.
YSL made substantial contributions to the conception and design of the manuscript, the acquisition of the data and drafted the paper; AGR participated in the design of the study and in the drafting of the paper; XYH treated and followed up the patient; ZYL undertook the CT examination and analyzed the photographs; DPM revised and finalized the manuscript.
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AKZ perform the examinations and analyzed data.
Study design: Umbilical, aortic and middle cerebral artery blood flow parameters were obtained by Doppler examination and retrospectively analyzed in 128 high-risk singleton pregnancies, followed by neurologic examination of the surviving children at 3 years of age.
Samples were obtained during surgical resection or endoscopic examination and were analyzed by immunohistochemistry on conventional tissue sections, given that this technique offers the advantage (over RT-PCR) of determining expression and cellular localization of the NIS protein instead of the NIS transcript[ 22].
Data were collected using face-to-face interview, clinical examination and laboratory investigation, and analyzed using SPSS version 13 software.
Written and practical examination scores were also collected and analyzed for all consenting students.
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