What exactly the replication profile of the Xi represents in terms of origin firing and usage and whether it plays a role in the initiation or maintenance of XCI still remain open questions.
However, what exactly regulates DNA replication in the regions where we observed a significant faster or slower replication (see Fig. 4) is not clear.
It remains unclear exactly why such replication is not possible, but functional constraints on receptor use in arthropods versus mammals provides one proximate explanation (van den Heuvel et al. 1999).
Redundancy, in our examples, is not just simple replication: even when QI is exactly reproducing the kinetics of MI, it is doing so through an intricately interconnected set of reactions.
While it is difficult to predict exactly how a replication-impaired phenotype might manifest, the survival of human patients with mutations in RECQL4 stands in contrast to studies on Drosophila, where deletion of the Sld2-like domain eliminated viability [ 21, 38, 42, 43].
(This is exactly what the semiconservative replication mechanism predicts).
He described the long-term fate of a modifier in infinite asexual populations, and showed that the error rate of DNA replication was exactly equal to the rate of environmental changes.
For example, the frequentist-based 95% confidence interval is often interpreted as meaning that there is a 95% chance that a parameter of interest lies between an upper and lower limit, whereas the correct interpretation is that 95 of 100 replications of exactly the same experiment capture the fixed but unknown parameter, assuming the alternative hypothesis about that parameter is true.
To confirm these results of increasing cell damage in the presence of high glucose levels, flow cytometry characterisation of cell death by apoptosis (Annexin V binding) and necrosis (Propidium Iodide marking) was performed on replications of exactly 10,000 cells for each of the three different insults.
In our replication, we use exactly the same variation and the same birth cohorts as Imbens and van der Klaauw (1995).
