Because both pCRP and mCRP proteins are derived from the same mRNA and because of questions concerning the exact specificity of antibody reagents used to immunochemically identify CRP antigens (Fig. 4; Samberg et al. 1988; Schwedler et al. 2003), many published reports should be interpreted with caution as to which conformational form of CRP was actually being synthesized in various tissue sites.
Exact specificity measurements are in Table S3.
Moreover, the exact specificity of the antibodies for variants of the NF54 RIFIN repertoire is not known.
Thus the exact specificity is not known, but Chromatin Immuno Precipitation (ChIP) at FLC and other loci in ashh2/sdg8/efs/ccr1 mutants compared to wt has suggested that ASHH2 is a H3K4 trimethyltransferase [6], [13] and/or a H3K36 di- and trimethyltransferase [7], [12], [14].
We determined that the exact specificity requirements were located in three regions of the sequence [ 129, 131].
The exact specificity of ALT for liver disease in NHANES is unknown because liver biopsies were not performed.
It has been suggested that determining the exact specificity of any inhibitor for either of the COX enzymes is frought with practical difficulties and may depend on the test and the laboratory that uses it [ 4].
Recent progress in sequencing and 3 D structure determination techniques stimulated development of approaches aimed at more precise annotation of proteins, that is, prediction of exact specificity to a ligand or, more broadly, to a binding partner of any kind.
Although the exact specificity and sensitivity of the original MaHV1 ELISA was not determined, it had been validated by testing of known MaHV1-positive (n = 14) and negative (n = 6) serum, and the assay correctly detected 100% of the positive samples and provided negative results for 100% of the negative samples (R. Eberle, pers. comm., 2013).
Due to the limited number of validated miRNA targets, the exact specificity and sensitivity of current determinants are unclear [ 20- 23]; however, estimates of precision of these determinants, alone or together, are typically reported to be about 50% at a sensitivity of 6-12% [ 24, 25], suggesting that sequence-based prediction methods are not fully capturing miRNA target preferences.
