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The molecular paleontology method of LTR-RT insertion dating has never claimed to provide rigorous and exact insertion time estimates because the pitfalls of using a single substitution rate for a population of LTR retrotransposons are well known (SanMiguel et al. 1998; Pereira 2004; Ma and Bennetzen 2006; Piegu et al. 2006).
To determine whether transgene chromosomal integration disturbed a known murine locus with oncogenic or suppressor gene function, we sought to localize the exact insertion site.
But based on current data, it is difficult to assign exact insertion sites before more genomic sequencing information is available because none of the insertion sequences including control has the exact match to tomato genomic sequences in GenBank.
We were unable to determine the exact insertion site by arbitrary PCR.
In this case, since there is no evidence for the exact insertion site location, the fragments are labeled as "approximate".
Further, the exact insertion site of the transposon, and thus the identity of the candidate gene(s) it disrupts, can be readily determined.
Similar(47)
Exact insertions which were found to be polymorphic in the human population were removed.
Thus, we caution that when complete genome data are available for Peramelemorphia or Notoryctemorphia allowing for a more exhaustive marker screen, it could reach other conclusions, as we cannot completely rule out the possibility of exact deletion or precise insertions of elements in our markers (van de Lagemaat et al. 2005).
It is often useful to determine the exact genomic insertion site for the transgene.
Molecularly mapping the exact FRT insertion site will also precisely define which genes are accessible for mosaic analysis.
Goodwin's classification [ 19] of screw head positioning was modified by the exact screw insertion.
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