Sentence examples for evolved variants from inspiring English sources
Evolved variants were vectorized and validated against existing hepatotropic serotypes.
Most pathogens have evolved variants that are able to express non-uniform surface structures.
Scaling-up the growth of the wild type and evolved variants to 50 mL in flask resulted in a lower cellulase activity production.
Here, we describe the directed evolution process in detail and the biophysical and crystallographic studies of the designed KE07 and its evolved variants.
The activity of the evolved variants, unlike the activity of the native R and TD recombinases, is suitable for genome engineering in Escherichia coli and mammalian cells.
Detailed analysis of these evolved variants and a few site-directed mutants generated de novo indicated three amino acid residues in the β1 domain are important for the improved protein folding yield.
A newly evolved variant, rAAV2-retro, perobustretrograderograccesscess to projection neurons with efficiency comparable to classical synthetic retrograde tracers and enables sufficient sensor/effector expression for functional circuit interrogation and in vivo genome editing in targeted neuronal populations.
In conclusion, the maximum polysaccharides conversion yield (12.4%%) was obtained for MIX A. The enzymes rCelStrep, rPoAbf or its evolved variant rPoAbf F435Y/Y446F were added alternatively or in combination to 15 mg/g glucan of MIX 1 (60 % Cellic® CTec3 and 40%% Cellic® HTec3 from Novozymes) for the hydrolysis reactions of pretreated NW.
On the other hand, the AFEX and EA pretreatments had comparable effects on the polysaccharides conversion yield after the hydrolysis of NW by using Cellic® CTec3 and HTec3 and the addition of rCelStrep, rPoAbf or its evolved variant rPoAbf F435Y/Y446F to the commercial mix improved the sacharification process.
The evolved variant performed better in E. coli than in human cells, suggesting optimal usefulness in bacterial rather than viral GDEPT vectors, and highlighting the influence of intracellular environs on enzyme function and the shaping of promiscuous enzyme activities within the "black box" of in vivo evolution.
We tested the ability of the GFP insertion reporters to distinguish between the insoluble wild type Rv2911 (putative penicillin-binding protein) from Mycobacterium tuberculosis (Mtb) H37Rv and a soluble evolved variant of Rv2911, previously engineered using the conventional C-terminal folding reporter (Waldo et. al., unpublished results).
