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This subfunctionalization may occur immediately after polyploidization or may arise over a longer period of evolutionary resolution [ 13, 14].
These and other investigations indicate that protist morphospecies usually offer too crude an evolutionary resolution to be ecologically informative [ 17- 21], reinvigorating the debate about at what level of taxonomic distinction are protists in fact cosmopolitan [ 22].
Despite potential recombination and convergence in the N- and C-terminal encoding regions, previous research found no conflict between the strongly supported nodes between separate N- and C-terminal trees and that concatenation of the terminal regions provides greater evolutionary resolution [ 12].
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The mode of molecular evolution presented here, in which diverse evolutionary resolutions exist at the level of a primary sequence that corresponds to a certain selective pressure on a protein property, may be relevant to various cases of adaptive modification in proteins, such as hydrophobic properties, molecular size, and electric charge.
However, the imaging entitlement of the new technology could equally be used to achieve very high SR if needed and from an evolutionary perspective, resolution improvement should be given high priority in the years ahead.
A possible evolutionary scenario for resolution of WGD is discussed.
In the eighties, D.N.A. sequencing and other techniques made it possible to create high-resolution evolutionary maps, known as phylogenies, that clearly showed that the ability to interbreed did not provide a good proxy for relatedness.
By combining historical analyses, like phylogenetic approaches, with high-throughput and high-resolution evolutionary experiments, we can observe parallel patterns of evolution that drive important phenotypic transitions.
Our high-resolution evolutionary study clarifies open questions regarding specific chromosomal regions that have long been studied in monocots.
Second, contigs with rosid species as the top hit were maintained for downstream filtering processes to identify sequences that could be useful for high-resolution evolutionary analysis.
In addition to the NGS application described here for one non-typhoidal Salmonella serovar, NGS has provided extraordinary insight into case studies involving: (i) traceback of tuberculosis infections in Canada [ 20]; (ii) high-resolution evolutionary linkage of global clones of Salmonella Typhi [ 19]; and (iii) identification of the origins of the Haitian Cholera outbreak [ 17] as a few examples.
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