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Briefly, despite sequence homology between avian and human isolates, the cross-species jump from birds to humans involves evolutionary barriers, including transmission bottlenecks and within-host adaptation.
These evolutionary barriers lead to avian precursors that have lower mortality in mice, less morbidity in ferrets, and lower viral titers in human epithelial cells (Belser et al., 2013; Watanabe et al., 2014; Zaraket et al., 2015).
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The relatively small number of specific amino acid substitutions that were required to rewire signalling in such synthetic circuits suggests that the evolutionary barrier to establish novel network connections in natural systems is relatively small.
The evolution of C4 photosynthesis occurred at least 62 times in 19 different families of the angiosperms (Sage et al., 2011), implying a low evolutionary barrier towards expression of this trait.
Another notable difference is that the physics-based model seems more residue-specific as it conserves fewer positions overall (36 vs 54), but those residues are typically more strongly conserved with larger co-evolutionary barriers to change.
Speciation arises from the evolutionary accumulation of barriers to successful reproduction between populations, so any process that accelerates trait evolution among populations along independent genetic trajectories will foster speciation and its maintenance.
We propose an evolutionary framework, the barrier theory of cancer, which is based on the distinction between barriers to oncogenesis and restraints.
It's this ballsiness and comfort in your own skin that we begrudgingly admire, even to the point where your gun laws, police brutality, and weird denial of evolutionary theory are no barriers to the thousands of us entering the Green Card Lottery every year.
They play an important evolutionary role as subcellular barriers restricting horizontal gene transfer and thereby providing microbial biodiversity.
However, phenotypic differences between isolates and broad scale comparative and molecular evolutionary analyses indicate multiple barriers that Rabies virus must overcome through adaptation.
Pseuduvaria species in New Guinea diversified morphologically in response to these physical and climatic barriers, with evolutionary shifts from unisexual into bisexual flowers, elongation of flowering and fruiting peduncles (favouring fruit-bat frugivory), and the evolution of distinct pollination systems [ 25, 27].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com