In other words, the evolution tends to stabilize the system.
Each run of the simulation began with 5,000 generations of evolution according to stabilizing selection, during which an initially genetically uniform population reached a mutation-drift-selection equilibrium.
We employ Proside, a method of directed evolution, to identify stabilized variants of Bs-CspB from Bacillus subtilis.
An evolution to a stabilized value of the surface stress, depending on the applied loads, was found.
In addition, protein engineering, particularly by directed or artificial evolution, has revealed mutations that serve to stabilize particular mesophilic enzymes of interest.
Amino acid substitutions rarely increase the stability of native proteins; hence, large libraries and high-throughput screens or selections are needed to stabilize proteins using directed evolution.
However, several processes act to stabilize resistance, including compensatory evolution where the fitness cost is ameliorated by additional mutation without loss of resistance, the rare occurrence of cost-free resistance mechanisms and genetic linkage or co-selection between the resistance markers and other selected markers.
Moreover, Shusta and colleagues were able to stabilize an scTCR by directed evolution using yeast surface display [3].
The postorbital septum (or bony closure behind the eye) in haplorrhine primates is thought to stabilize the eye slightly, allowing for the evolution of the fovea.
Additionally, there is a selective pressure to stabilize the stem-loop structure and some structural changes are tolerated [ 51], which may result in the evolution of robustness.
