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This measure was performed for the TEV-7DA, TEV-PC2 and TEV-PC76 at the first and last evolution passages.
In our experiments we have performed short-term evolution passages measuring the virus accumulation, virulence and infectivity rate.
Restriction enzymes Eco81I and EcoT14I were used to check which viral genotype was present at each evolution passages.
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Virus accumulation per gram of infected tissue was assessed and equal numbers of 20 LFU used to initiate the next evolution passage.
To investigate the potential for nucleotide polymorphisms to contribute to TLO gene evolution during passaging, we analyzed the TLO sequences for acquisition of new SNPs during passage.
A C. glutamicum strain was engineered by using a combination of gene deletions and adaptive evolution with 70 passages of growth-based selection.
Because the previous long-term experimental evolution with serial passages lacked explicit size selections, the selection target and its pressure were uncontrolled, allowing for the accumulation of mutations unrelated to size changes.
This same mutation has also been previously reported in serial-passage evolution experiments of TEV in N. tabacum: in 10% of lineages after 15 one-week passages (Bedhomme et al. 2012), and in 30% of lineages after three 9-week passages (Zwart et al. 2014).
With DSC imaging, images are repeatedly acquired to trace the signal evolution following the passage of intravenously administered paramagnetic contrast agent.
Although the zoonotic potential of CWD is considered low, identification of multiple CWD strains and the potential for agent evolution upon serial passage hinders a definitive conclusion.
Sequences whose sites have experienced large numbers of substitutions, either as a function of a rapid rate of molecular evolution or the passage of long periods of evolutionary time, will likely exhibit a high frequency of homoplasious character states.
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