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The evolution of the sol, mainly the chemical structure, during the processes of hydrolytic condensation and organic polymerisation was studied as a function of the sol concentration through Fourier transformed infrared spectroscopy (FTIR), rheometry, laser diffraction analysis and contact angle.
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The acetone formation gene (adc), as a part of the sol operon, exhibited highly-coordinated expression with the other sol genes.
solB (sCAP_176), located just upstream of the sol operon, has been identified as the putative repressor of the sol operon and thus of solventogenesis [ 84].
To further confirm expression changes of the sol operon, qRT-PCR analysis was performed.
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