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This relatively small number of fungal-conserved clusters reflects the large evolutionary distance between members of the fungal kingdom, as well as complex patterns of gene gains and losses during the evolution of fungi.
The identification of leuA in Phycomyces adds to the growing body of evidence that some primary metabolic pathways or parts of them have arisen multiple times during the evolution of fungi, probably through horizontal gene transfer events.
Moreover, HTs seem to play an important role in the evolution of fungi [ 29, 72- 75].
Physiological variations are the result of the adaptation and evolution of fungi, considering their hosts, original habitats, and other factors.
Evolutionary characteristics of CYP51 and CYP61 could provide the important information on evolution of fungi and CYPomes.
It suggests that CYP51 and CYP61 are evolutionarily conserved, which could be used to trace the evolution of fungi.
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Recent initiatives, particularly the UNICORN project, have facilitated the sequencing of several opisthokonts related to the last common ancestor of the metazoans and fungi, providing an interesting window into the evolution of both fungi and animals [ 53- 56].
This suggests that host adaptation has driven the evolution of filamentous fungi.
Gene deletion is also likely to have played role in the evolution of discrete fungi, as exemplified by the PPIase repertoire differences of C. glabrata and S. cerevisiae after their divergent post-WGD evolution (Table 16 [See Additional File 1]).
UmFKBP2 and YlFKBP1 are found on the earliest branch, suggesting that these are the earliest precursors of this group, which does not agree with the evolution of the fungi as both are members of different taxa.
In addition, the protein families of these regulators and the protein families of CAZys and secondary metabolism-related enzymes have recently expanded in the evolution of filamentous fungi, (Pezizomycotina)[ 40].
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