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The significance of gene fusion in eukaryotic evolution has been previously demonstrated [19].
Especially for the trpA and trpB genes of these species, an increased rate of evolution has been previously postulated [ 25].
Integration of extrachromosomal elements that accounts for replicon evolution has been previously proposed in Aeropyrum pernix (Robinson and Bell 2007).
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Exon-intron framework properties such as length distribution and GC content evolution have been previously used to demonstrate the gene evolution [ 44].
Most of the amino acid sites detected to be under adaptive evolution have been previously identified to have important functional roles.
Codon-specific models for classical molecular evolution have been previously described by Goldman et al. [ 46] and Mayrose et al. [ 47].
The influence of gene length on sequence evolution had been previously reported for Populus tremula [ 38], stressing the importance of accounting for all diverse determinants of levels of gene and protein evolution.
A detailed description of the experimental evolution populations has been previously published (Chandler et al. 2012).
The construction of the populations and experimental evolution design has been previously detailed (Teotonio et al., 2012).
The yeast display levels of scFv-intein proteins could potentially be improved via directed evolution, as has been previously reported for a variety of proteins.
The evolutionary situation for Tom22 is outlined above, and although a Tom20 receptor protein is present in plants, it represents a case of convergent evolution that has been previously well described [ 27, 54, 55], thus, plant and yeast Tom20 proteins are not orthologous.
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