Sentence examples for evolution assay from inspiring English sources
For the physiologically-relevant hydrogen evolution assay, methyl viologen and sodium dithionite were replaced by NADPH (1 mM).
One emerging technology that shows promise for screening libraries with remarkable efficiency is miniaturization of the directed evolution assay into artificial reaction compartments with cell-like dimensions.
All statistical analyses were run in the R environment version 2.11.1 for most analyses, version 3.0.1 for body size and reversed experimental evolution assay, available at www.r-project.org [ 53].
As an alternative to microfluidic droplet sorting, we introduce a straightforward method to convert a directed evolution assay previously conducted in water-in-oil emulsion droplets into double emulsions in two separate microfluidic devices at a rate of about 10 droplets per hour.
Residual enzyme activities were measured using the MV-linked H2-evolution assay.
performed evolution, enzyme assays, point mutation reversions, and growth curve analysis.
DOI: http://dx.doi.org/10.7554/eLife.00631.003 The experiment outlined in Figure 1 requires a protein for which it is possible both to reconstruct the natural evolution and to assay for the functions that contribute to biological fitness.
The project will involve classic microbiological techniques (including bacteria and phage plating), experimental evolution, plant infection assays, and use of the novel droplet digital PCR technique to quantify phage and bacterial replication in the plant environment.
We provide a historical perspective of the evolution of the neurosphere assay and highlight limitations in the use of sphere-forming assays in the context of neurospheres.
Here, we use directed evolution and a multiplexed assay to screen a library of >14,000 members to generate three derivatives of the antibacterial compound, andrimid.
The conditions that support the generation of myeloid and erythroid cells from CFCs were developed early in the evolution of the CFC assay.
