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Values of dN − dS<0, = 0 or >0 indicate negative selection, neutral evolution and positive selection, respectively.
The dN/dS ratios of <1, = 1 and >1 indicate purifying selection, neutral evolution and positive selection on the protein involved, respectively.
We propose that extensive diversification of the het-d and het-e genes is achieved by a unique combination of high mutation supply, concerted evolution and positive Darwinian selection.
ω values <1, = 1, and >1 indicate purifying selection, neutral evolution, and positive selection, respectively.
In humans, signals of rapid evolution and positive selection were also detected.
Homonyms may be useful in detecting convergent evolution and positive selection in protein evolution.
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This ratio is denoted ω, with ω = 1, <1 and >1 indicating neutral evolution, purifying and positive selection, respectively [28].
The results of a likelihood ratio test between I) the M1a (nearly neutral evolution) and M2a (positive selection) models, and II) the M7 (nearly neutral evolution) and M8(β+ωs>1) (positive selection) models implemented by the codeml program from the PAML package [24] are shown in Table 2.
The MIC values generated range between 0, indicating independent evolution, and a positive value whose magnitude depends on the amount of covariation.
This is consistent with the fact that relatively rarely used isoforms are more likely to be species-specific and the evidence for faster evolution, and more positive selection in alternative regions compared to constitutive ones [ 21- 25].
Using both pairwise d N/d S values and the results of a branch-site model, we find evidence for elevated rates of amino acid evolution and increased positive selection in genes expressed exclusively in adult somatic tissues as compared with genes expressed in larvae or ovaries.
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