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Of 25 precursor loci chosen on the basis of extensive RNA-Seq coverage (see Additional File 1: Supplemental Table S4), 18 showed evidence of transcript splicing and 8 of alternative splicing, suggesting that post-transcriptional modification of miRNA transcripts is likely to be widespread.
Overall, these validation experiments further suggest that mRNA-Seq can provide strong evidence of transcript expression.
Transcriptomic studies have also provided evidence of transcript similarity at the mRNA level [25].
This idea is further supported by evidence of transcript overexpression in metastatic lesions of prostate cancer (Dorkin et al, 2000).
A recent transcriptomic study analyzing differential gene expression during thermal stress in M. faveolata also provides evidence of transcript decreases of genes involved in calcification [ 29].
Of over 12,000 putative retrocopy-derived genes that arose mainly in the primate lineage, 726 with strong evidence of transcript expression were examined in detail.
Similar(48)
Nuclear RNA samples derived from WT cells showed some evidence of transcripts close to the TSS (site a) and transcripts of pre-mRNA (site b) but barely detectable spliced transcripts (site c) in contrast to Lsh−/− samples (Fig. 5C).
Our RT-PCR results, wherein evidence of transcripts for both PKD2L1 and PKD1L3 was detected in human fungiform papillae of normal subjects (Figure 2), are consistent with our immunohistochemical results.
In contrast, the other species show no evidence of transcripts of the expected size.
However, there is now evidence of transcripts spanning chromosomal regions larger than 2 Mb.
We analysed the data from Kaida et al for evidence of transcripts that are reduced by 2 or more fold following SSA treatment.
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