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Exact(7)
Cells were then rinsed with dH2O, air dried, and visualized macroscopically for evidence of staining.
Sectioning revealed the stained substance to be in the lumen of the intestinal bulb, with no evidence of staining in intestinal bulb epithelial cells (Fig. 2B).
This staining pattern was detected with the other antibodies to c19orf10, whereas there was no evidence of staining with control antibodies.
There was no relationship between staining and stage, node status or size but more poorly differentiated carcinomas had evidence of staining (36%) than well (17%) or moderately (14%) differentiated carcinomas (P = 0.02).
The reactivity was graded as "0" if there was no evidence of staining, "1+" if 1 25% of cells stained, "2+" if 26 49% stained, "3+" if 50 74% stained, and "4+" if >75% of cells stained [ 32].
Specimens for fascin, K8 and β4-integrin immunostaining were divided into three categories: High (homogenous positive tumor in more than 50% of cells or/and high membranous and cytoplasmic staining in more than 50% of cells; ++/+++); low 20-500% positive tumor cells; +); negative (less than 20% positive cells or no evidence of staining; -).
Similar(53)
The positive B19 control sample gave clear positive staining, whereas the negative controls showed no evidence of IHC staining.
Nasa has now revealed that there is evidence of stain-marks on Mars's canyons and crater-walls: water is trickling downhill before drying up in the valleys and plains.
The weighted score was obtained by multiplying the staining intensity score ranging from 3+ (strongest positive) to 0 (no evidence of stain) and the score for the percentage of positive cells ranging from 3+ (100% stained) to 0 (no cells stained).
The weighted score is obtained by multiplying the staining intensity score (3+, strong positive stain in most cells; 2+, moderate stain in cells; 1+, weak stain in cells; 0, no evidence of stain) and score for the percentage of positive cells (3+, most of cells stained; 2+, half of cells stained; 1+, few cells stained; 0, no cells stained).
No TRAP-positive staining cells were found in the control joints (Fig. 3a), but there was evidence of TRAP staining at the synovium bone interface in joints treated with OSM, IL-1 or TNF-α (Fig. 3b,3c,3d, respectively).
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Justyna Jupowicz-Kozak
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