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Electron microscopy provides direct evidence for binding of the dimer to F-actin and indicates that it binds to three monomers along the long-pitch helix of the actin filament.
(A) EMSA-based evidence for binding of E. coli fabA promoter to FadR protein of three origins.
To provide direct evidence for binding of RMS-P3/RR to furin, we reasoned that furin-overexpression should result in increased phage RMS-P3/RR binding.
Approaches including also 'weaker' putative binding sites with very low ChIP-seq coverage did however so far not provide evidence for binding sites diverging significantly from the GAS motif [3], [12].
There is evidence for binding and uptake of heparin by Kupffer cells [ 27].
On average, the selected TFs had evidence for binding 20% of genes per target module.
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As our ITC measurements reported here provided little evidence for such binding selectivity, we repeated the binding assays with FP assays.
124 Antihuman IgG antibodies were used to detect IVIg, and there was evidence for brain binding, but the experiment did not reveal specific binding to Aβ plaques.
The results corroborate evidence for these binding interactions and indicate multiple high-affinity modes of binding.
Docking simulations provided corroborating evidence for these binding interactions and indicated multiple high-affinity modes of binding.
The data represent bioinformatics analysis for key RNA-binding proteins interactions with intronic AREs and provide experimental evidence for HuR binding to AREs.
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