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To determine SCV migration relative to the Golgi region, the positions of the centroids corresponding to DsRed and MannII-EGFP fluorescence were calculated in each image of every time-lapse sequence.
Images were collected by time lapse every 68 or 26 milliseconds using Metamorph software and converted to multiframe TIFF images.
Video clip S-2 (003.mov): Time lapse images (every 5 min for 60 min and then every 15 min for the next 60 min) of the movement of neutrophils from the vascular channels to the tissue compartment of the fluidic assay.
Time lapse photography (every 20 seconds) was carried out for 36 h.
For both probing schemes, fluorescence images are collected every 2 min using automated time lapse imaging controlled via Metamorph.
Time lapse images were acquired every 30 seconds in both the FITC and DIC channels.
Practice every day, and take pictures or a time lapse to see your improvement!
Time lapse setting.
Why the long time lapse?
Roger Zare's "Time Lapse" alluded to two techniques from photography: time-lapse and high-speed.
Matt Week – Day Two Time Lapse – Music by MGMT.
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