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Every third section was stained with cresyl-violet.
To quantify the volume of the striatum, the area of the entire striatum was measured in every third section.
Every third section was incubated with primary antibodies at 4°C overnight, and then labeled with Alexa488- or Alexa568-conjugated goat secondary antibody (1 300, Invitrogen, Carlsbad, CA).
To count the number of regenerating neurons, every third section was selected from each ipsilateral and contralateral DRG (L4 and L5) from each animal.
To assess the number of PH3-positive cells and BrdU-positive cells, every third section of the entire hippocampus and all sections of the subventricular zone were analyzed.
The number of Nissl-stained motor neurons in the sciatic motor pool of every third section (n = 60) between the L2 and L5 levels of the spinal cord were counted.
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FB labeled neurons on every third sections were counted.
Every fourth section was collected for free-floating IHC.
To avoid double counting of neurons, every second section was omitted.
Cells were counted in every sixth section.
Every fourth section was stained with H&E.
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