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Every tenth section was stained with 1% cresyl violet.
Graft volume and cell density of the graft were determined measuring every tenth section of the graft.
In order to obtain a systematic sample without bias throughout the papillae, each papilla was exhaustively sectioned and every tenth section was saved onto a slide.
The serial sections from each ovary were stained with hematoxylin and picric acid methyl blue, and every tenth section was analyzed for the number of healthy (non-atretic) primordial, primary and small preantral follicles, as described [26], [28], [68], [69].
The follicles were counted in every tenth section using the oocyte nucleus as a marker and classified according to the shape and number of layers of somatic cells surrounding the oocyte [31].
To calculate taste bud size, the perimeters of the taste bud from every tenth section were outlined and the corresponding area was computed by Zeiss LSM image browser [16].
Similar(24)
4G8 immunohistochemistry and ThS staining were quantified in every tenth sections, in a total of 5 sections.
Every sixth section covering the entire DG was processed for BrdU immunohistochemistry.
Total cell numbers were estimated by multiplying the number of cells counted in every sixth section by six.
An investigator blind to treatment counted every sixth section throughout the dentate gyrus for the total number of DCX-positive neurons and for those with tertiary dendrites.
Every fourth section was collected for free-floating IHC.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com