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Instead of using a fixed reference, we utilize the combination of the above aligned samples to generate dynamic reference for every samples.
More than 1000 genes have a rpbp > 10 in every samples.
Then, we computed the fraction of adenylated and uridylated mapped reads for each mature microRNA in every samples.
Following primers were used: for Rsad2 AAGCTGAGGAGGTGGTGCAG and GAAAACCTTCCAGCGCACAG, and for Colec11 TGGACAACCAGGTCACTCAA and AGCCTGTGCCAGGTATGAAG. Triplicates of every samples were measured following an amplification protocol including a 10 minutes activation step at 95°C and 40 cycles à 15 s at 95°C, 20 s at 60°C and 40 s at 72°C.
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"I am sure that Fina and the doping authorities have taken every sample they can take," Leonard said.
Coveney said veterinary staff were examining the plant's records with a view to identifying ingredients from every sample tested.
Every sample was dipped three times.
Tth for every sample in recorded traces.
Field estimate is calculated after every sample.
Every sample measurement was repeated 15 times.
However, soil SAR increased at every sampling date after application.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com