To validate the gene expression profiles, qPCR analyses were performed for four genes (CG11212, CG6234, CG1225 and CG17957) at selected developmental stages, using independently isolated batches of total RNA extracted from staged embryos.
Isolated batches of 200 cells were grown in DMEM (Gibco) supplemented with 1× Glutamax (Gibco), 120 μg/ml penicillin, 100 μg/ml streptomycin, and 20% heat-inactivated FCS (Gibco) on 12-well culture plates (CELLSTAR, Greiner bio-one, Kremsmünster, Austria) at 37°C under 5% CO2 in the absence or presence of TGF-β1 (10 ng/ml) (R&D Systems).
Proteoglycans were isolated by batch anion-exchange on Q-Sepharose (GE Healthcare), followed by HiTrap-Q and eluted with NaCl (0.5M 1.5M).
RNA was isolated from batches of ∼106 cells using the RNeasy Mini Kit (Qiagen; www.qiagen.com).
Nuclei were isolated in batches of 24 for amplification.
This chapter investigates the possibility of using a passively or actively controlled platform to isolate a batch of high tech equipment from micro vibration of the floor of a building subject to nearby traffic-induced ground motion.
This paper investigates the possibility of using a microvibration control platform to isolate a batch of high tech equipment from the floor of a building subject to nearby traffic-induced ground motion.
Herein, bacterial community composition was investigated as an isolated variable within batch biofilm reactors via comparison of PPCP removals for three distinct inocula.
The ability of Chlamydomonas to survive in the absence of light (and photosynthesis) meant that he could identify mutants with faulty photosynthesis pathways, mutants that in Arabidopsis or another model system would likely die before they could be isolated within a batch.
The WSSV-TW strain was isolated from a batch of WSSV-infected Penaeus monodon collected in Taiwan in 1994 [5], [50], and it was used as the template for amplification of the vp19, vp24, vp26, vp28 and vp51A coding regions in all of the following experiments.
We also measured for isolated clones in batch, by fitting the growth curve during the exponential phase.
