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Alternatively, BLAT tended to over-insert large gaps in the alignments because it suspected every insertion or deletion in the sequence to potentially be the start of an intron.
Similarly on-the-nose choices crop up through the Bikini section – as when an animated film-production slate claps in sync with a Reich percussion slap, just before every insertion of period documentary footage.
That's due to the fact that the tracking software is actually built into the ad-serving software, so every insertion is recorded and accounted for.
The cloning strategy that we devised was based on 'recycling' unique RE recognition sites through repeated destruction and replacement with every insertion event (Figure 1).
After every insertion, the model was refilled with water and checked microscopically for any enclosed air bubbles.
If the size of every insertion is 6.8 kb (Sha et al. 2000), the present-day S. melliferum would have a genome size of 3.6 Mb, of which 70% comprised plectroviral fragments.
Similar(52)
These results show that, like other TLS pols, PrimPol makes a mistake copying undamaged template DNA about once every 100 2500 insertion events.
One of the puzzles of the transgene assays for PREs is that silencing does not occur at every chromosomal insertion site.
Our earlier studies of pol β replication with various stereospecific BPDE-adducted templates were performed with nongapped DNA where synthesis was distributive (i.e., polymerase would dissociate from the DNA substrate after every nucleotide insertion).
However, not every Ds insertion will cause trap events or show reporter expression within the first 7 d after fertilization, and many protein traps are also likely to be out of the reading frame and may not produce mCherry fusion proteins.
We quantified the PrimPol misinsertion frequency on undamaged DNA templates and find that the fidelity of polymerization is indeed comparable to other TLS pols (1 mistake every ∼100 2500 insertion events).
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