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A control loop was used to re-initialize the population every five generations.
Analyses were run for 100,000 generations and sampled every five generations, with a burn-in of 50,000.
Cell aliquots were saved every five generations on a cold plate and then pelleted and frozen for genomic DNA extraction.
We grew samples of the barFLEX pool in medium containing glucose (repressing) or galactose (inducing) and then collected samples every five generations for measurement of relative strain abundance.
Each lab population evolved for a maximum of 50 generations and nematode samples were collected every five generations for mtDNA analysis.
In order to prevent cells from entering stationary phase, throughout the experiment, we performed batch dilutions by monitoring optical density and diluted cultures (1 10,000) at approximately every five generations (10 hours).
Similar(51)
It moves one cell vertically and one horizontally every four generations, giving the impression that it is crawling across the screen.
Speaking of design, it also looks like the iPhone will get a fresh coat of paint, following in the tradition of changing things up considerably every two generations.
Trees were sampled every hundred generations and the consensus tree was estimated by using a burn in of 3,000 trees.
One colony (Xen-R) was selected every two generations under the same conditions described above in order to maintain selection pressure.
Trees were sampled every thousand generations.
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