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The calibration point was based on three Horowitz samples with well-documented genealogies coalescing 402 ybp.
This prompted us to use this node as an internal calibration point.
We followed our previous studies on the porcini mushrooms25 to select the calibration point.
The average of these values is used as a calibration point for 0 tilt.
We ran an additional analysis using a fossil calibration point on the basal node of Caribbean Cyrtognatha clade.
It is now our calibration point.
We determine the sensitivity of focal divergence times to specific calibration points by jackknife approach in which each calibration point is excluded from analysis.
Calibration point A exhibited the largest SS.
Each of the calibration points used was individually assessed in separate analyses to compare the appropriateness of the other calibration point, and thereby determining the ratio (or fit) of calibration points.
Apparently the long phylogenetic distance of calibration point A from the Burmanniaceae clade or the influence of calibration points in more closely related clades reduces the impact of this calibration point.
Then, by using one calibration point at a time, and one branch path emanating from the chosen calibration point, we obtained the divergence time estimates.
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