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The data represented with mean ± SD from two assays and three replicates in every assay.
For the screen, amikacin was included in the positive control wells in every assay plate.
Buffer, protein and substrate blanks were measured for every assay and were subtracted from the experimental data.
Purified VTG from Atlantic salmon (Salmo salar) was used as a positive control within every assay run.
Therefore to enable cross-assay comparison, the top 20% of compounds (by activity or pIC50) were considered active so that every assay would have the same hit rate.
This is because nearly every assay in PubChem is different from others in terms of various factors, not just underlying pharmacology or binding geometry.
Every Assay Commission passed the coinage that it was called upon to examine.
Efficiency corrections were made for each gene in every assay.
A non-template control (NTC) was run with every assay.
TGFβ1 standard curves were undertaken for every assay.
For every assay, the cells were incubated in the respective hormones for 48 hours.
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CEO of Professional Science Editing for Scientists @ prosciediting.com