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In the present study, we were able to demonstrate effects of DHEA on the expression of every adhesion molecule investigated.
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Note that length values in vitro and in silico are not comparable – the FilaQuant software processing florescence microscopy images has cutoff parameters (regarding length and thickness of lines to consider a filament) while for the simulation every integrin/focal adhesion with at least one bound actin is considered a filament.
In every other respect adhesion forces measured with cantilever 3 were consistent with the other two cantilevers.
Special care was taken to achieve high spatial resolution to determine the forces transmitted at every single putative adhesion structure.
A total of 5000 pretreated cells (see above) were added to the E-plates and xCELLigence was programmed to monitor adhesion every minute for 240 min.
The P. falciparum strain FCR3CSA was selected for the adhesive phenotype every three weeks by adhesion to plastic flasks coated with 10 mg ml−1 CSA as previously described (Viebig et al., 2005).
Cells were grown for 24 h, with impedance measured every minute during 14 h (adhesion phase), then every 15 min.
The plate, which contained gold microelectrodes on its bottom, was monitored every ten minutes for four hours (adhesion process), then once every 30 min until the end of the experiment, (72h).
The plate containing gold microelectrodes on its bottom was monitored every 10 min for 4 h (adhesion process), then once every 30 min, until the end of experiment, for a total of 72 h.
The family structure (pedigree) was available after a demographic census performed for every volunteer at his adhesion in the project.
Every substrate induced cell adhesion and spreading, indicating successful integrin-ligand interactions with all the different compounds.
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