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The challenged piglets were monitored for clinical signs of disease every 3 hrs.
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Weaning procedure lasted 20-24 hours with changes in parameters noted every 2-3 hrs.
The samples at three nicotine levels (28.33, 118.67 and 458.26 μg mL−1) were analyzed in triplicate every 8 hrs within 24 hrs.
For bupropion, 150.0 mg was taken orally every 12 hrs by three healthy human volanteers for 14 days.
Media was changed every 24 hrs.
Feces were collected for 4 days every 24 hrs.
Culture media and reagents were replaced every 24 hrs.
Mice were treated 24 hrs prior to infection and every 48 hrs thereafter, unless otherwise stated.
Larvae were transferred every 12 hrs to queen cups with new food (200 µl per cup).
Parasite morphology was evaluated by examination of blood smears every 6 hrs until reinvasion, i.e. 52 hrs post synchronization.
The worms on all these plates were scored for survival every 24 hrs.
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