For chemical treatment, -jasmonic acid or abscisic acid (Sigma-Aldrich) at a concentration of 0.3 mM or 50 μM, respectively, in aqueous solution, or ethephon (0.3 mM, Sigma-Aldrich) at a concentration of 3 mM in sodium phosphate buffer (50 mM, pH7), was evenly sprayed (about 3 ml) onto intact plants.
NF155-turbo green fluorescent protein (turbo GFP -transfected and naive HEK293 cells were evenly mixed and resuspended in Dulbecco's modified EaGFP -transfectedtandinaive fetal bovine serum and 1 mmol/L etHEK293diaminetetraacells acid (EDTA) (FCM buffer) at a concentration of 1.0 × 10 cells/mL, and rotated at 4°C for 60 min.
Biomass was harvested by centrifugation, washed in 1× PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4), and split evenly into different tubes containing 50 mM Tris HCl (pH 7.0), 0.01% yeast extract, chloramphenicol (100 µg/ml), and an individual substrate at a final concentration of 5 mM.
Make 1 or 2 light dots in the circle, evenly spread at a right angle to your baseline.
Had the methane measured by Mumma spread out evenly over the planet, then TLS should have detected a concentration of 6 parts per billion, he says.
Precipitation is fairly evenly distributed throughout the year, with an annual average of 56 inches (1,420 mm) and a concentration on the coast.
In the Naples sketches, the light falls evenly at the front, as on a curtain.
PpIX quenches the tryptophan fluorescence evenly at all wavelengths and shows a maximal quenching close to 100%.
Like a concentration camp.
