The pooled CH2Cl2 phases and the residual 40% (v/v) methanol phase were separately evaporated to remove the respective solvent in a rotary evaporator (40°C), and the residues were each weighed to give the yield of the CH2Cl2 and methanolic extracts, respectively.
The extract from each step was evaporated to dryness separately and redissolved in distilled water before use.
The extracts were combined (separately for each samples), filtered, evaporated to dryness using a rotary evaporator, and then lyophilized.
But it quickly evaporated when the two negotiators appeared separately, and with cursory statements, after the meeting at Lancaster House, an 18th-century mansion in central London that is often used for diplomatic talks.
Analyses were conducted separately for each study replication (UHT, evaporated, and powdered milk).
Two 2 ml extracts from the methanol/water phase or non-polar samples from the chloroform phase were separately collected to new tubes and then evaporated to dryness.
The aqueous suspension left after partitioning was evaporated to dryness and the residue was sonicated further with acetone and methanol separately for 20 min, thrice each.
