Two hours later, echography and mean arterial pressure (MAP) evaluation were performed; blood samples and heart were then collected to evaluate biological parameters (lactate, troponin T, creatinine), inflammation, autophagy and total O-GlcNAcylation by western blot.
Color and sensory evaluation were performed after drying.
RNA isolation, sample processing, and microarray analyses (Agilent Technologies), as well as statistical evaluation, were performed by Arrows Biomedical GmbH (Muenster, Germany).
Eight weeks after surgery, immunofluorescence staining, histologic morphometrical analysis and electrophysiologic evaluation were performed.
Three-dimensional analysis using micro CT and histological evaluation were performed.
Clinical scoring, digital photography, and RCM evaluation were performed at 72 hours, and RCM images were subjected to blinded evaluation.
Clinical and laboratorial assessments, with CD4+ cell count and CT evaluation, were performed and compared among groups I and II.
Thermodynamic analysis and economic evaluation were performed based on a typical 1000 MW bituminous coal fired power plant incorporating the proposed LTPD concept.
The spectra acquisition and evaluation were performed with the Spectra 5.3 software.
Pathogen inoculation and disease evaluation were performed as previously described by Zhu et al. (2012).
Qualitative, histomorphometric evaluation were performed of histological sections of two specimens from the same patient.
